Introduction
Circulating cell-free DNA (cfDNA) analysis often requires enrichment of short DNA fragments while minimizing contamination from high-molecular-weight genomic DNA. In plasma samples, background genomic DNA released from leukocytes can significantly interfere with downstream molecular analysis.
The MagPure Circulating DNA Rich Mini Kit is designed as a complementary workflow for circulating DNA studies. The system applies a selective magnetic bead purification strategy to remove large DNA fragments and enrich circulating DNA molecules from plasma or serum samples. The enriched cfDNA can be directly used for downstream applications such as PCR, quantitative PCR, biochip analysis, and sequencing workflows.
For primary plasma extraction, refer to:
· Column system → (HiPure Circulating DNA Kit – IVD3182)
· Magnetic system → (MagPure Circulating DNA Maxi Kit – IVD5435)
Details
Specifications
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Features
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Specifications
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Main Functions
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Rich small fragment cfDNA from 0.6ml serum plasma, remove DNA fragments＞500bp
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Applications
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qPCR, NGS, etc.
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Purification technology
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Magnetic beads technology
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Process method
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Manual or automatic
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Sample type
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Serum, plasma
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Sample amount
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0.6ml
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Elution volume
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Time per run
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Principle
The purification workflow is based on a sequential magnetic bead binding strategy that separates DNA fragments according to size.
Following protein digestion and lysis, magnetic particles with strong affinity for high-molecular-weight DNA are first introduced to selectively capture large genomic DNA fragments. These particles are removed during magnetic separation, effectively reducing contamination from long DNA molecules.
In the subsequent step, a second type of magnetic particle captures the remaining fragmented DNA molecules present in the supernatant. These particles selectively bind circulating DNA fragments typically present in plasma samples, allowing enrichment of cfDNA fractions suitable for downstream analysis.
For detailed engineering characteristics and validation data, refer to the core circulating DNA extraction system
Kit Contents
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Contents
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1292750
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12927200
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Purification Times
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50 preps
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200 preps
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MagPure Particles F
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1.2 ml
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4.5 ml
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MagBind Particles
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1.2 ml
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4.5 ml
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Proteinase K
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24 mg
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90 mg
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Protease Dissolve Buffer
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1.8 ml
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10 ml
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Buffer MLB
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30 ml
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120 ml
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Buffer GDP
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15 ml
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60 ml
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Buffer MAW1
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40 ml
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250 ml
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Buffer MW2*
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20 ml
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50 ml
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Elution Buffer
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15 ml
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60 ml
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Storage and Stability
Proteinase K, MagBind Particles and MagPure Particles F should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
Purchase Guide
For guidance on selecting the most appropriate DNA extraction system based on sample type, input volume and workflow requirements:
👉 Magen Kits Selection Guide
For a broader technical overview of cfDNA workflow routes, processing logic and downstream application orientation:
👉 cfDNA Extraction & Enrichment Workflows
For reference workflow structure and comparative processing logic across representative cfDNA workflows:
👉 cfDNA Workflow Notes (IVD3182 / IVD5435 / 12927)
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