Introduction
This kit is designed to rich and extract 100bp-500bp circulating DNA from 5 ml cell-free body fluids (such as plasma, serum), and remove fragments above 500bp. Machine reaction only takes 90 minutes. Magnetic-particle technology provides high-quality DNA that is suitable for direct use in downstream applications such as PCR and next generation sequencing.
Designed to integrate with the Magen circulating DNA platform, this kit complements both membrane-based and magnetic bead-based extraction systems.
For primary extraction workflows, see:
· Column system → HiPure Circulating DNA Kit – IVD3182
· Magnetic system → MagPure Circulating DNA Maxi Kit – IVD5435
Details
Workflow
Workflow Overview
The MagPure Circulating DNA Rich Maxi Kit introduces a fragment selection–based workflow for cfDNA enrichment. Instead of recovering total circulating DNA directly, the workflow first removes high-molecular-weight DNA and then selectively captures shorter cfDNA fractions through a staged particle-based process.
Sample Handling Logic
The workflow is based on sequential size-selective binding. Under optimized conditions, large DNA fragments are preferentially removed, while shorter cfDNA remains in solution for subsequent capture. This design enables improved representation of shorter fragments and reduced genomic DNA background.
Time and Workflow Characteristics
Due to the additional size-selection stage, enrichment workflows typically require longer handling time compared to standard extraction. Under typical conditions, the workflow is completed within about 110–140 minutes, depending on input volume and selection conditions. For detailed selection parameters, workflow steps and processing guidance, please refer to the Workflow Note in the Download section.
Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by elution buffer.
Application Fit Summary
The following table summarizes workflow situations where 12927 may be considered for short-fragment enrichment. It is intended as an application-fit guide rather than a summary of published clinical validation.
|
Application Fit
|
Sample / cfDNA Need
|
Why 12927 Fits
|
|
Fragmentomics / MCED-oriented workflows
|
Plasma cfDNA workflows where short-fragment composition or size distribution is analytically important
|
Enriches short cfDNA fragments, typically around 100–500 bp, while reducing larger genomic DNA background
|
|
Short-fragment-sensitive cfDNA methylation workflows
|
cfDNA methylation studies where fragment size distribution may influence assay sensitivity or marker interpretation
|
Helps concentrate shorter circulating DNA fractions when methylation assay design benefits from size-selected input
|
|
Exploratory early detection research
|
cfDNA samples with elevated large-fragment genomic DNA background concern
|
Provides a size-selective enrichment route when routine total cfDNA extraction is not sufficient for downstream short-fragment analysis
|
Note: 12927 is intended for short-fragment enrichment workflows rather than routine total cfDNA extraction. Whether size selection is appropriate depends on the downstream assay, study design and whether fragment distribution itself is part of the analytical signal.
Kit Contents
|
Contents
|
1292750
|
12927200
|
|
Purification Times
|
50
|
200
|
|
MagPure Particles G
|
20 ml
|
80 ml
|
|
MagBind Particles (selection particles)
|
14 ml
|
58 ml
|
|
Selection Solution
|
100 ml
|
400 ml
|
|
Proteinase K
|
300 mg
|
1.2 g
|
|
Protease Dissolve Buffer
|
25 ml
|
100 ml
|
|
Buffer SDS(20%)
|
15 ml
|
60 ml
|
Buffer MLK
|
300 ml
|
3 x 450 ml
|
|
Buffer BST1
|
225 ml
|
2x 450 ml
|
Buffer MKW1
|
225 ml
|
2x 450 ml
|
Buffer MW2*
|
50 ml
|
2x 100 ml
|
Buffer AE
|
10 ml
|
30 ml
|
Storage and Stability
MagPure Particles G, MagBind Particles and Proteinase K should bestored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored dry at roomtemperature (15–25°C) and are stable for at least 18 months underthese conditions.The entire kit can be stored at 2–8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
Purchase Guide
For guidance on selecting the most appropriate DNA extraction system based on sample type, input volume and workflow requirements:
👉 Magen Kits Selection Guide
For a broader technical overview of cfDNA workflow routes, processing logic and downstream application orientation:
👉 cfDNA Extraction & Enrichment Workflows
For reference workflow structure and comparative processing logic across representative cfDNA workflows:
👉 cfDNA Workflow Notes (IVD3182 / IVD5435 / 12927)
Related cfDNA Application Notes: These application notes help explain how this circulating DNA extraction route fits different plasma cfDNA research workflows and downstream assay requirements.
👉 Application Map · Methylation Biomarkers · Fragmentomics & MCED
