MagPure Pathogen DNA/RNA Enrich Kit

MagPure Pathogen DNA/RNA Enrich Kit is designed for enrichment of pathogen nucleic acids from complex biological samples where host nucleic acids are present in large excess. In many clinical samples such as blood, plasma or respiratory specimens, microbial and viral genomes may represent only a small fraction of the total nucleic acids, which can reduce pathogen detection sensitivity in sequencing-based analysis.

This system combines host nucleic acid reduction with magnetic bead purification to improve recovery of pathogen DNA and RNA from samples with low microbial abundance. The workflow increases the relative proportion of pathogen nucleic acids prior to sequencing preparation while maintaining compatibility with automated nucleic acid extraction platforms.

Within the Magen pathogen extraction portfolio, this kit is commonly used for sequencing-oriented pathogen detection workflows where host background nucleic acids may interfere with pathogen signal. For workflows requiring direct pathogen nucleic acid extraction without enrichment, laboratories may use MagPure Pathogen DNA/RNA Kit B (R6672B).

Specifications

Extraction Principle

Complex biological samples often contain abundant host DNA released from lysed cells, while microbial and viral genomes are present at much lower levels. To address this imbalance, the MagPure Pathogen DNA/RNA Enrich Kit incorporates a host nucleic acid reduction step prior to pathogen nucleic acid purification.

During the initial treatment stage, DNase digestion selectively degrades exposed host DNA. Microbial and viral nucleic acids remain protected within intact cell walls or viral particles during this step. Subsequent chemical lysis releases pathogen nucleic acids, which are then captured by magnetic beads under chaotropic binding conditions and purified through washing and elution steps.

Engineering Features

The system integrates host nucleic acid reduction with magnetic bead purification in a workflow compatible with automated extraction platforms.

Key engineering elements include controlled DNase digestion conditions to reduce host DNA background, optimized bead-binding chemistry for efficient recovery of fragmented nucleic acids, and stable purification performance from larger input volumes. Reagents are processed to minimize background nucleic acids, helping reduce potential interference from reagent-derived DNA in sequencing workflows. These features support pathogen sequencing applications where host or environmental DNA can otherwise dominate sequencing reads.

Kit Contents

Storage and Stability

Proteinase K, DNase I powder and Particles MPN9 should be stored at 2–8°C upon arrival. However, short-term storage (up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.

For guidance on selecting the most appropriate DNA extraction system based on sample type, input volume and workflow requirements:

👉 Magen Kits Selection Guide