Total RNA Purification Kit with DNase Treatment

Introduction

The HiPure Total RNA Plus Kit is designed for RNA purification workflows requiring stringent removal of genomic DNA contamination. The system integrates a DNA removal column with DNase digestion to ensure efficient elimination of residual genomic DNA during RNA purification.

The workflow supports purification of total RNA including small RNA species from tissue samples and cultured cells. The protocol is commonly used in RNA-seq experiments, RT-PCR analysis and other applications where trace genomic DNA contamination may affect downstream results.

For routine RNA extraction workflows laboratories may refer to the HiPure Total RNA Plus Kit (R4111), while automated RNA purification systems can be implemented using the MagPure Universal RNA Kit (IVD3020).

Details

Specifications

Features	Specifications
Main Functions	Isolation total RNA（miRNA）from tissue, cell using two columns and DNase plus reagent
Applications	RT-PCR, cDNA synthesis, second generation sequencing
Products	RNA, miRNA
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Clinical tissues, cells, lymphocytes
Sample amount	Tissue: <20 mg Cells: <5 x 10⁶
Yield	2-50μg
Elution volume	≥30μl
Time per run	≤25 minutes

Kit Contents

Contents	IVD4121
Purification Times	50 Preps
HiPure DNA Mini Column Ⅱ	50
HiPure RNA Mini Columns	50
2ml Collection Tubes	150
Proteinase K	24 mg
Protease Dissolve Buffer	1.8 ml
DNase I	600 μl
DNase Buffer	6 ml
Buffer RTL	40 ml
RNA Digestion Buffer	15 ml
Buffer RWC*	20 ml
Buffer RW2*	20 ml
Nuclease Free Water	10 ml

Storage and Stability

Proteinase K should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, Proteinase K up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.

Purchase Guide

Select the right purification kit to get impactful results：

Ⅰ. Purchase guide of Magen kits based on sample

Ⅱ. Cross purchase guide for Magen kits vs Other brands

For any customized product or OEM service, please contact us.

CAT NO	PRODUCT NAME	SIZE	PRICE
IVD4121	HiPure Total RNA Kit	50 preps	$348.00

HiPure Total RNA Kit

Introduction

Details

Purchase Guide

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