Introduction
DNA extraction from FFPE tissue samples can be challenging due to the presence of crosslinked proteins, pigments, and residual fixation reagents that may inhibit downstream molecular assays.
The MagPure FFPE DNA Kit (High Pure) (D6323D) is developed to provide enhanced purification performance for FFPE genomic DNA isolation using magnetic bead technology. The binding chemistry combines optimized salt conditions with alcohol-mediated adsorption to improve removal of contaminants commonly present in FFPE tissue extracts.
This purification strategy supports recovery of highly purified DNA suitable for PCR, qPCR, and other inhibitor-sensitive molecular assays where DNA purity is critical for reliable amplification.
For laboratories focusing on sequencing workflows where fragment distribution control may be beneficial, the MagPure FFPE DNA Kit (D6323B) provides an alternative magnetic bead system designed to manage small DNA fragment recovery during purification.
Column-based purification workflows are available through the HiPure FFPE DNA Kit (D3126), which represents the membrane-based FFPE DNA extraction system in the Magen product portfolio.
For applications requiring simultaneous extraction of both DNA and RNA from FFPE tissue sections, the MagPure FFPE DNA/RNA Kit (R6327) provides a co-extraction workflow using magnetic bead adsorption chemistry.
Details
Specifications
|
Features
|
Specifications
|
|
Main Functions
|
Isolation high pure total DNA from FFPE using high bind beads
|
|
Applications
|
PCR and viral DNA detection, etc.
|
|
Purification technology
|
Magnetic beads technology
|
|
Process method
|
Manual or automatic
|
|
Sample type
|
Paraffin embedded tissue samples
|
|
Sample amount
|
1-6 slices of 10-20μm
|
|
Elution volume
|
≥30μl
|
|
Time per run
|
≤60 minutes
|
Extraction Principle
Samples are first deparaffinized and digested with Proteinase K to release nucleic acids from the FFPE tissue matrix.
Following digestion, DNA purification can be performed using two alternative binding strategies depending on sample characteristics.
In the high-salt binding mode, a chaotropic binding buffer promotes selective adsorption of DNA while improving removal of pigments and polysaccharides commonly present in FFPE samples.
In the alcohol-mediated binding mode, ethanol is introduced during the adsorption step to enhance DNA binding efficiency and improve overall DNA recovery.
This dual-binding design allows the purification workflow to be adapted according to sample composition and DNA quality.
After washing steps to remove residual contaminants, purified DNA is eluted in a low-salt buffer suitable for downstream molecular workflows.
Kit Contents
|
Contents
|
D632301D
|
D632302D
|
|
Purification Times
|
48 Preps
|
96 Preps
|
|
MagPure Particles N
|
1.1 ml
|
2.5 ml
|
|
RNase A
|
10 mg
|
20 mg
|
|
Proteinase K
|
24 mg
|
48 mg
|
|
Protease Dissolve Buffer
|
3 ml
|
6 ml
|
|
Buffer DPS
|
60 ml
|
100 ml
|
Buffer ATL
|
15 ml
|
30 ml
|
Buffer BST1
|
30 ml
|
60 ml
|
Buffer BW1
|
13 ml
|
44 ml
|
Elution Buffer
|
15 ml
|
30 ml
|
Storage and Stability
Proteinase K, RNase A and MagPure Particles N should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Purchase Guide
For guidance on selecting the most appropriate FFPE nucleic acid extraction system based on target analyte, workflow format and downstream application requirements:
👉 FFPE Nucleic Acid Extraction Purchase Guide
For a broader technical overview of FFPE DNA, RNA and DNA/RNA co-extraction workflow routes, processing logic and application-oriented route design:
👉 FFPE Nucleic Acid Extraction Workflows Explained
For detailed workflow structure, estimated processing time and route-specific handling logic across representative FFPE workflows:
