The HiPure Cell miRNA Kit provides a column-based workflow for purification of total RNA including microRNA from cultured cells and various animal and human tissues, including difficult-to-lyse tissues samples.

RNA molecules including small RNA species are captured by the silica membrane and purified through sequential washing and elution steps. The workflow supports microRNA expression profiling and RT-PCR analysis.

For workflows requiring enrichment and separation of miRNA and large RNA fractions laboratories may use the HiPure Universal miRNA Kit (R4310).

Specifications

Principle

Biological samples are first lysed and homogenized in a highly denaturing guanidine isothiocyanate-containing buffer, which immediately inactivates DNases and RNases to ensure isolation of intact DNA and RNA. The lysate is then passed through a Mini spin column. This column, in combination with the high-salt buffer, allows selective and efficient binding of genomic DNA. Flow-through from the column is digested by Proteinase K in the presence of ethanol. This optimized digestion, together with the subsequent addition of further ethanol, allows appropriate binding of total RNA, including miRNA, to the column. Contaminants are efficiently washed away and high-quality RNA is eluted.

Advantages

• High quality - one step RNA extraction reagent combined with silica gel column can obtain the highest concentration
• Fast - several samples can be extracted in 40 minutes
• High applicability - samples including animals, plants, bacteria, cells, etc.
• High concentration - efficiently remove macromolecular RNA, enrich small RNA and improve sensitivity
• Safe - no phenol chloroform extraction and no use of Trizol reagent

Kit Contents

Storage and Stability

Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

Choosing the appropriate RNA extraction kits depends on sample type, RNA species and workflow requirements.

For detailed guidance across tissue and cell RNA extraction workflows, refer to the Tissue/Cell RNA Kits Selection Guide.