Introduction
MagPure FFPE RNA Kit enables purification of RNA from paraffin-embedded tissue samples using magnetic bead adsorption technology. Magnetic particle separation provides flexible processing for laboratories handling multiple FFPE samples or integrating nucleic acid extraction with automated liquid handling systems.
Magnetic bead workflows also simplify handling of fragmented RNA molecules typically present in archived FFPE tissues.
For column-based RNA purification workflows see HiPure FFPE RNA Kit - R4143.
Laboratories requiring simultaneous purification of DNA and RNA from FFPE tissues may refer to MagPure FFPE DNA/RNA Kit - R6327.
This kit is suitable for high-throughput extraction of total RNA from paraffin embedded tissue samples, including miRNA. The high-throughput extraction can be realized by 96 hole magnetic frame and 96 holeo scillator, various nucleic acid extractors (such as KingFisherFle, MagMix32, MagMix 96) or cooperating with the pipette workstation (Hamilton, Tican) for automatic extraction.
Details
Specifications
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Features
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Specifications
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Main Functions
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Isolation total RNA from FFPE tissue
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Applications
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RT-PCR, cDNA synthesis, second generation sequencing
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Products
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RNA, miRNA
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Purification method
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Polydisperse magnetic beads
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Purification technology
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Magnetic beads technology
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Process method
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Manual or automatic
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Adaptive instrument
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Nucleic acid extractor, pipetting workstation
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Sample type
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FFPE slice, FFPE embedded tissue
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Sample amount
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≤6 slices
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Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Technical Validation
MagPure FFPE RNA Kit was evaluated as a magnetic bead-based RNA extraction workflow for FFPE tissue samples. In the test report, 5 mg one-year paraffin-embedded chicken liver and fish liver samples were processed using the MagPure FFPE RNA workflow, followed by Nanodrop measurement and agarose gel electrophoresis analysis. From 5 mg FFPE chicken liver input, the kit produced RNA yields of 9.5–12.2 µg under the 0.7V isopropanol condition and 19.6–20.7 µg under the 1.5V condition. From 5 mg FFPE fish liver input, RNA yields were 5.8–8.3 µg under the 0.7V condition and 7.6–11.1 µg under the 1.5V condition. The workflow includes magnetic particle binding, DNase I treatment, magnetic washing and elution, supporting both manual handling and automated magnetic extraction platforms such as KingFisher Flex or similar systems.
Kit Contents
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Contents
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IVD3022
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Purification Times
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200 Preps
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MagBind Particles
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4.5 ml
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Proteinase K
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100 mg
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Protease Dissolve Buffer
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6 ml
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DNase I
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4 x 600 µl
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DNase Buffer
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30 ml
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Buffer DPS
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200 ml
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Buffer FRL
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40 ml
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Buffer AL
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40 ml
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Buffer MW1*
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110 ml
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Buffer MW2*
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2 x 50 ml
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Nuclease Free Water
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30 ml
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Storage and Stability
Proteinase K and MagBind Particles should be stored at 2–8°C upon arrival. DNase I should bestored at -20°C. However, short-term storage (DNase I up to 1 weeks, Proteinase K and MagBind Particles up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under theseconditions.
Purchase Guide
For guidance on selecting the most appropriate FFPE nucleic acid extraction system based on target analyte, workflow format and downstream application requirements:
👉 FFPE Nucleic Acid Extraction Purchase Guide
For a broader technical overview of FFPE DNA, RNA and DNA/RNA co-extraction workflow routes, processing logic and application-oriented route design:
👉 FFPE Nucleic Acid Extraction Workflows Explained
For detailed workflow structure, estimated processing time and route-specific handling logic across representative FFPE workflows:
