HiPure Total RNA Plus Kit

The HiPure Total RNA Plus Kit (R4111) is designed for purification of high-quality total RNA from animal tissues and cultured cells using silica membrane spin column technology. The system integrates an additional genomic DNA removal step that eliminates genomic DNA contamination prior to RNA purification, enabling reliable RNA preparation for downstream gene expression analysis.

The extraction workflow combines efficient sample lysis with silica membrane RNA adsorption to selectively purify RNA molecules while removing proteins, genomic DNA and other contaminants. The resulting RNA is suitable for RT-PCR, transcriptomic analysis and other RNA-based molecular biology workflows.

Within the Magen RNA extraction systems, the HiPure Total RNA Plus Kit serves as a core column-based solution for high-purity RNA preparation when removal of genomic DNA contamination is critical for downstream applications.

Specifications

Engineering Characteristics

Integrated genomic DNA removal

The workflow includes a dedicated gDNA removal column that eliminates genomic DNA contamination before RNA purification, reducing the need for additional DNase treatment.

Efficient RNA binding chemistry

Silica membrane adsorption enables selective binding of RNA molecules while cellular proteins and other contaminants are removed during washing steps.

Optimized lysis and inhibitor removal

The extraction buffers efficiently disrupt cellular structures and remove contaminants that may interfere with downstream reverse transcription reactions.

Spin-column workflow

The purification procedure is compatible with routine laboratory centrifuges and supports rapid RNA purification workflows in research laboratories.

Technical Validation

HiPure Total RNA Plus Kit was evaluated as a rapid silica column-based total RNA extraction workflow with integrated genomic DNA removal. The kit uses a DNA Mini Column before RNA binding, enabling total RNA purification from cultured cells and animal tissues without phenol / chloroform extraction or isopropanol precipitation. The purified RNA is intended for downstream applications such as RT-PCR, Northern blotting, poly(A)⁺ RNA purification, nuclease protection and in vitro translation.

In protein- and lipid-rich tissue testing, RNA was extracted from 10 mg chicken liver and 10 mg chicken brain samples. From 10 mg chicken liver input, the kit produced RNA yields of 45.46–48.24 µg, with A260/280 values of 2.11–2.12 and A260/230 values of 1.75–2.23. From 10 mg chicken brain input, RNA yields were 4.29–4.55 µg, with A260/280 values of 2.13–2.18 and A260/230 values of 1.90–2.15 under the tested conditions.

A comparison with a reference column-based RNA extraction workflow was performed using chicken liver input amounts of 5 mg, 10 mg, 20 mg and 40 mg. With 40 mg chicken liver input, R4111 produced RNA yields of 147–156 µg, compared with 147 µg from the reference workflow. With 20 mg input, R4111 produced 95–98 µg RNA, compared with 84 µg from the reference workflow. With 10 mg input, R4111 produced 41–47 µg RNA, compared with 37 µg from the reference workflow, while 5 mg input produced 19–21 µg RNA under the tested conditions.

Across the tested chicken liver input range, R4111 maintained A260/280 values of approximately 2.11–2.15 and A260/230 values of approximately 2.07–2.28. Agarose gel electrophoresis showed intact RNA band patterns and no obvious genomic DNA contamination, supporting the effectiveness of the gDNA removal column for routine DNA reduction before RNA purification.

The kit was also evaluated in a rapid cultured-cell extraction workflow using 5 × 10⁶ cultured cells per sample. Six cell samples were processed in approximately 30 minutes, and electrophoresis analysis showed clear total RNA bands with good integrity. These results support R4111 as a fast total RNA extraction workflow for routine animal tissue and cultured-cell samples where reduced genomic DNA background and short hands-on processing time are required.

Typical Applications

Total RNA purified using the HiPure Total RNA Plus Kit is suitable for a wide range of RNA analysis workflows, including:

Related Products

• HiPure Universal RNA Kit (R4130) – purification of total RNA including small RNA fractions using MagZol reagent chemistry

• MagPure Universal RNA Kit (IVD3020) – magnetic bead-based automated purification of RNA from tissue and cultured cells

• MagPure Blood RNA Kit (R6611) – magnetic bead purification of RNA from whole blood samples

• HiPure Universal miRNA Kit (R4310) – enrichment and purification of small RNA molecules from tissue samples

Kit Contents

Storage and Stability

HiPureKit can be stored dry at room temperature (15-25°C) and are stable for at least18 months under these conditions. During shipment, crystals or precipitationmay form in the Buffer RLC. Dissolve by warming buffer to 37°C.

Choosing the appropriate RNA extraction kits depends on sample type, RNA species and workflow requirements.

For detailed guidance across tissue and cell RNA extraction workflows, refer to the Tissue/Cell RNA Kits Selection Guide.