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MagPure Circulating DNA Maxi Kit IVD5435

CAT NO PRODUCT NAME SIZE PRICE
IVD5435 MagPure Circulating DNA Maxi Kit 50 preps $497.00

Introduction

This Kit is designed forpurification of high quality circulating DNA (cfDNA) from 1-6ml cell-free bodyfluids (such as plasma, serum). The purified DNA is suitable for direct use indownstream applications such as PCR, real-time PCR, Biochip analysis and NGS.

Details


Specifications

Features Specifications
Main Functions Isolation circulating DNA from 1-6ml plasma, serum, body fluids
Applications qPCR, NGS, etc.
Purification technology Magnetic beads technology
Process method Manual or automatic
Sample type Serum, plasma
Sample amount 1-6ml
Elution volume ≥50μl
Time per run ≤60 minutes


Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by elution buffer.

Advantages

  • Economy - less than 50% of the price of Qiagen and other imported products
  • Automatic - without labour
  • High purity - OD 260 / 280 : 1.7-1.9, OD 260 / 230 :1.5-2.0


Kit Contents

Contents IVD5435
Purification Times 50
MagPure Particles F 11 ml
Carrier RNA 310 μg
Proteinase K
220 mg
Protease Dissolve Buffer
15 ml
Buffer SDS
15 ml
Buffer MLK 400 ml
Buffer MAW1 250 ml
Buffer MW2 50 ml
Elution Buffer 60 ml

Storage and Stability

Proteinase K, Carrier RNA and MagPure Particles F should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.


Experiment Data





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